Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
MilliporeSigma™ PIPES, Sodium Salt, Molecular biology grade, OmniPur™, Calbiochem™,
CAS: 100037-69-2 Molecular Formula: C16H33N4Na3O12S4 Molecular Weight (g/mol): 670.665 InChI Key: FPDACHMSOMILQI-UHFFFAOYSA-K PubChem CID: 3086462 IUPAC Name: trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+]
| PubChem CID | 3086462 |
|---|---|
| CAS | 100037-69-2 |
| Molecular Weight (g/mol) | 670.665 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)[O-].C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+].[Na+] |
| IUPAC Name | trisodium;2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonate;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate |
| InChI Key | FPDACHMSOMILQI-UHFFFAOYSA-K |
| Molecular Formula | C16H33N4Na3O12S4 |
PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical
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CAS: 10010-67-0 Molecular Formula: C8H17N2NaO6S2 Molecular Weight (g/mol): 324.34 MDL Number: MFCD00065472 InChI Key: OGGAIRCLBMGXCZ-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1
| CAS | 10010-67-0 |
|---|---|
| Molecular Weight (g/mol) | 324.34 |
| MDL Number | MFCD00065472 |
| SMILES | [Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1 |
| IUPAC Name | sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate |
| InChI Key | OGGAIRCLBMGXCZ-UHFFFAOYSA-M |
| Molecular Formula | C8H17N2NaO6S2 |
MES, Monohydrate, cGMP Manufactured, 99.5%, Spectrum™ Chemical
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CAS: 145224-94-8 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 213.25 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N IUPAC Name: 4-(2-sulfonatoethyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CC[NH+]1CCOCC1
| CAS | 145224-94-8 |
|---|---|
| Molecular Weight (g/mol) | 213.25 |
| SMILES | [O-]S(=O)(=O)CC[NH+]1CCOCC1 |
| IUPAC Name | 4-(2-sulfonatoethyl)morpholin-4-ium |
| InChI Key | SXGZJKUKBWWHRA-UHFFFAOYSA-N |
| Molecular Formula | C6H13NO4S |
Thermo Scientific Chemicals AMPSO, 0.2M buffer soln., pH 9.0
CAS: 102029-60-7 Molecular Formula: C7H16NNaO5S Molecular Weight (g/mol): 249.257 MDL Number: MFCD00067500 InChI Key: UVGYCHPTWHWEPZ-UHFFFAOYSA-M PubChem CID: 44119996 IUPAC Name: sodium;2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonate SMILES: CC(C)(CO)NCC(CS(=O)(=O)[O-])O.[Na+]
| PubChem CID | 44119996 |
|---|---|
| CAS | 102029-60-7 |
| Molecular Weight (g/mol) | 249.257 |
| MDL Number | MFCD00067500 |
| SMILES | CC(C)(CO)NCC(CS(=O)(=O)[O-])O.[Na+] |
| IUPAC Name | sodium;2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonate |
| InChI Key | UVGYCHPTWHWEPZ-UHFFFAOYSA-M |
| Molecular Formula | C7H16NNaO5S |
Gibco™ DPBS, calcium, magnesium, glucose, pyruvate
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | Sodium Pyruvate |
|---|---|
| Physical Form | Liquid |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Sterility | Sterile-filtered |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco |
Bicine, 99.38%, MP Biomedicals™
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N, N-bis [2-hydroxyethyl]glycine PubChem CID: 8761 ChEBI: CHEBI:40957 SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | N, N-bis [2-hydroxyethyl]glycine |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | GTE buffer |
| TSCA | Yes |
| Recommended Storage | Keep cold |
MOPS, 99%
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N Synonym: 4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid PubChem CID: 70807 ChEBI: CHEBI:44115 SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| Synonym | 4-Morpholinepropanesulfonic acid; 3-(4-Morpholinyl)propanesulfonic acid |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Thermo Scientific Chemicals Potassium phosphate, 0.2M buffer soln., pH 7.4
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
| CAS | 7778-77-0 |
|---|---|
| Molecular Weight (g/mol) | 136.08 |
| MDL Number | MFCD00011401 MFCD00147253 |
| SMILES | [K+].OP(O)([O-])=O |
| IUPAC Name | potassium dihydrogen phosphate |
| InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
| Molecular Formula | H2KO4P |
MES, >98%, MP Biomedicals™
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-[N-Morpholino]ethanesulfonic acid PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.248 |
| MDL Number | MFCD00149409 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| Synonym | 2-[N-Morpholino]ethanesulfonic acid |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Gibco™ HBSS (10X), no calcium, no magnesium, phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Physical Form | Liquid |
|---|---|
| Recommended Storage | Ambient temperatures |
| Concentration | 20X |
Thermo Scientific Chemicals TRIS, 0.5M buffer soln., pH 8.5
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MOPSO Sodium Salt, MP Biomedicals™
CAS: 79803-73-9 Molecular Formula: C7H14NNaO5S Molecular Weight (g/mol): 247.24 InChI Key: WSFQLUVWDKCYSW-UHFFFAOYNA-M IUPAC Name: sodium 2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonate SMILES: [Na+].OC(CN1CCOCC1)CS([O-])(=O)=O
| CAS | 79803-73-9 |
|---|---|
| Molecular Weight (g/mol) | 247.24 |
| SMILES | [Na+].OC(CN1CCOCC1)CS([O-])(=O)=O |
| IUPAC Name | sodium 2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonate |
| InChI Key | WSFQLUVWDKCYSW-UHFFFAOYNA-M |
| Molecular Formula | C7H14NNaO5S |